說明
The elasmobranch targeted eDNA project aim to enumerate elasmobranch diversity using eDNA Metabarcoding across eastern Indonesia. A primary goal of the project is to produce a comprehensive database on shark species occurrences in areas around Savu seas, Nusa Tenggara to support current government efforts to save sharks Population. To date we have collected more than 10 water samples from MPA areas around Savu Seas using tools designed to collect water samples (sterivex, and Niskin water sampler). The project is a collaboration between Biodiversitas Indonesia and Misool Foundation. As of now, the DNA reads collected from the samples have no Elasmobranch detected, nonetheless we still publish the taxa that were detected from the samples while working on possible eDNA marker that most suitable to detect elasmobranch species.
資料紀錄
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 2,063 筆紀錄。
亦存在 1 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。
此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Anggoro A (2023). Savu Sea-Indonesia eDNA Dataset. Version 1.97. BIONESIA. Occurrence dataset. https://cloud.gbif.org/asia/resource?r=edna_id_2022&v=1.97
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 BIONESIA。 This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: bbb81807-fc4c-4e4e-bc1c-2caee84a4a06。 BIONESIA 發佈此資源，並經由Participant Node Managers Committee同意向GBIF註冊成為資料發佈者。
關鍵字
Occurrence; Observation
聯絡資訊
- 內容提供者
- Program Development Consultant
- Jl. Buluh Indah No.122, Pemecutan Kaja, Kec. Denpasar Utara
http://orcid.org/https://orcid.org/0000-0002-5482-4123
- 內容提供者
- Program Development Consultant
- Jl. Buluh Indah No.122, Pemecutan Kaja, Kec. Denpasar Utara
- 擁有者 ●
- 連絡人
- Director
- Jl. Buluh Indah No.122, Pemecutan Kaja, Kec. Denpasar Utara, Kota Denpasar, Bali 80118
- 內容提供者
- Jl. Buluh Indah No.122, Pemecutan Kaja, Kec. Denpasar Utara, Kota Denpasar, Bali 80118
地理涵蓋範圍
The Savu Sea is a small sea within Indonesia named for the island of Savu (Sawu) on its southern boundary. It is bounded by Savu and Rai Jua to the south, the islands of Rote and Timor (split between East Timor and Indonesia) to the east, Flores and the Alor archipelago to the north/northwest, and the island of Sumba to the west/northwest. Between these islands, it flows into the Indian Ocean to the south and west, the Flores Sea to the north, and the Banda Sea to the northeast.
| 界定座標範圍 | 緯度南界 經度西界 [-10.509, 120.234], 緯度北界 經度東界 [-8.418, 123.816] |
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分類群涵蓋範圍
DNA sequences from water samples will be identified to species whenever possible. When sequences were unable to be assigned to a taxonomic name, a dedicated OTU (Operational Taxonomic Name)/occurenceID will be given to the sequences
| Kingdom | Bacteria |
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計畫資料
The proposal aims to enrich existing 2000 coral reef fish database species found in Eastern Indonesia, by particularly focusing on elasmobranch, one of the species that are heavily targeted in global fisheries industries. Major activities will include comprehensively list the group using the most updated eDNA metabarcoding technique and train conservationists to use molecular data for conservation purposes. An important target for the project is to develop a practical field guidebook to collect and analyze eDNA data for research and conservation purposes. The combined activities will contribute massively for education, research and marine conservation activities in the region.
| 計畫名稱 | Sawu-Sea Indonesia eDNA Metabarcoding Project |
|---|---|
| 辨識碼 | BIFA6_021 |
| 經費來源 | Biodiversity Information Fund for Asia (BIFA) Miscellaneous awards given to BIONESIA and Misool Foundation to conserve Sharks in Indonesia |
| 研究區域描述 | Taman Nasional Perairan Laut Sawu or Savu Sea National Marine Park is located in East Nusa Tenggara Indonesia covering an area of more than three million hectares. The hallmark of the marine park is its unique feature as a migratory area for whale species and is a home for a variety of marine megafauna. It houses 11 whale species, 7 dolphin species, 5 manta, and Turtle species and includes two sunfish and dugongs. |
| 研究設計描述 | The sampling collection event summarized the collection event across 4 locations in Savu Sea waters. We collected surface seawater samples in 5 sites from each location. eDNA sample from sea water sample were collected across 4 designated locations across Sawu Sea, with 5 sites in each location. 2 liter of sea water sample were collected with sterile nalgene bottle from sea surface in every site. Water sample than filtered through a 0.22-133 micron Sterivex™ filter. eDNA samples and blanks will be extracted using the DNeasy Blood & Tissue Kit (QIAGEN, Hilden, 143 Germany). Extracted DNA was then sent to sequencing facility for NGS metabarcoding method using Illumina platform. |
參與計畫的人員:
取樣方法
The sampling collection event summarized the collection event across 4 locations in Savu Sea waters. We collected surface seawater samples at 5 sites from each location. eDNA samples from seawater sample were collected across 4 designated locations across the Sawu Sea, with 5 sites in each location. 2 liters of seawater samples were collected with sterile Nalgene bottle from the sea surface in every site. Water sample than filtered through a 0.22-133 micron Sterivex™ filter. eDNA samples and blanks will be extracted using the DNeasy Blood & Tissue Kit (QIAGEN, Hilden, 143 Germany). Extracted DNA was then sent to a sequencing facility for the NGS metabarcoding method using the Illumina platform.
| 研究範圍 | Taman Nasional Perairan Laut Sawu or Savu Sea National Marine Park is located in East Nusa Tenggara Indonesia covering an area of more than three million hectares. The hallmark of the marine park is its unique feature as a migratory area for whale species and is a home for a variety of marine megafauna. It houses 11 whale species, 7 dolphin species, 5 manta, and Turtle species and includes two sunfish and dugongs. For this study, we specifically choose areas from Solor, Rote, Adonara, Manggarai, and West Manggarai to collect the water samples. The areas have never been studied before and hence the biodiversity information from the area will important for biodiversity management and conservation |
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方法步驟描述:
- 1. For each of the sampling sites, one water samples were collected an homogenized 2. DNA was extracted from each of the samples with a modified protocol based on PowerMax soil kit (MoBio, now Qiagen) 3. For each DNA extract 1 µl, the COI region was amplified in triplicates with the primers mlCOIintF and jgCOIR, using Amplitaq Gold. 4. All PCR products were built into Illumina sequencing libraries with the TruSeq PCR free kit. 5. Sequencing of the 10 samples was done with the Illumina MiSeq platform using the V2 kit 250 bp paired end sequencing. 6. Paired reads were demultiplexed and analyzed with Metaworks https://github.com/terrimporter/MetaWorks using cutadapt, vsearch and dada2, and vsearch. 7. Sequences were annotated by comparison with the Ribosomal Database classifier v2.13 (RDP classifier)
額外的詮釋資料
| 替代的識別碼 | bbb81807-fc4c-4e4e-bc1c-2caee84a4a06 |
|---|---|
| https://cloud.gbif.org/asia/resource?r=edna_id_2022 |