Descripción
The elasmobranch targeted eDNA project aim to enumerate elasmobranch diversity using eDNA Metabarcoding across eastern Indonesia. A primary goal of the project is to produce a comprehensive database on shark species occurrences in areas around Savu seas, Nusa Tenggara to support current government efforts to save sharks Population. To date we have collected more than 10 water samples from MPA areas around Savu Seas using tools designed to collect water samples (sterivex, and Niskin water sampler). The project is a collaboration between Biodiversitas Indonesia and Misool Foundation. As of now, the DNA reads collected from the samples have no Elasmobranch detected, nonetheless we still publish the taxa that were detected from the samples while working on possible eDNA marker that most suitable to detect elasmobranch species.
Registros
Los datos en este recurso de registros biológicos han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 2.063 registros.
también existen 1 tablas de datos de extensiones. Un registro en una extensión provee información adicional sobre un registro en el core. El número de registros en cada tabla de datos de la extensión se ilustra a continuación.
Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.
Versiones
La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.
¿Cómo referenciar?
Los usuarios deben citar este trabajo de la siguiente manera:
Anggoro A (2023). Savu Sea-Indonesia eDNA Dataset. Version 1.97. BIONESIA. Occurrence dataset. https://cloud.gbif.org/asia/resource?r=edna_id_2022&v=1.97
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es BIONESIA. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento-NoComercial (CC-BY-NC 4.0).
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: bbb81807-fc4c-4e4e-bc1c-2caee84a4a06. BIONESIA publica este recurso y está registrado en GBIF como un publicador de datos avalado por Participant Node Managers Committee.
Palabras clave
Occurrence; Observation
Contactos
- Proveedor De Contenido
- Program Development Consultant
- Jl. Buluh Indah No.122, Pemecutan Kaja, Kec. Denpasar Utara
http://orcid.org/https://orcid.org/0000-0002-5482-4123
- Proveedor De Contenido
- Program Development Consultant
- Jl. Buluh Indah No.122, Pemecutan Kaja, Kec. Denpasar Utara
- Propietario ●
- Punto De Contacto
- Director
- Jl. Buluh Indah No.122, Pemecutan Kaja, Kec. Denpasar Utara, Kota Denpasar, Bali 80118
- Proveedor De Contenido
- Jl. Buluh Indah No.122, Pemecutan Kaja, Kec. Denpasar Utara, Kota Denpasar, Bali 80118
Cobertura geográfica
The Savu Sea is a small sea within Indonesia named for the island of Savu (Sawu) on its southern boundary. It is bounded by Savu and Rai Jua to the south, the islands of Rote and Timor (split between East Timor and Indonesia) to the east, Flores and the Alor archipelago to the north/northwest, and the island of Sumba to the west/northwest. Between these islands, it flows into the Indian Ocean to the south and west, the Flores Sea to the north, and the Banda Sea to the northeast.
| Coordenadas límite | Latitud Mínima Longitud Mínima [-10,509, 120,234], Latitud Máxima Longitud Máxima [-8,418, 123,816] |
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Cobertura taxonómica
DNA sequences from water samples will be identified to species whenever possible. When sequences were unable to be assigned to a taxonomic name, a dedicated OTU (Operational Taxonomic Name)/occurenceID will be given to the sequences
| Reino | Bacteria |
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Datos del proyecto
The proposal aims to enrich existing 2000 coral reef fish database species found in Eastern Indonesia, by particularly focusing on elasmobranch, one of the species that are heavily targeted in global fisheries industries. Major activities will include comprehensively list the group using the most updated eDNA metabarcoding technique and train conservationists to use molecular data for conservation purposes. An important target for the project is to develop a practical field guidebook to collect and analyze eDNA data for research and conservation purposes. The combined activities will contribute massively for education, research and marine conservation activities in the region.
| Título | Sawu-Sea Indonesia eDNA Metabarcoding Project |
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| Identificador | BIFA6_021 |
| Fuentes de Financiación | Biodiversity Information Fund for Asia (BIFA) Miscellaneous awards given to BIONESIA and Misool Foundation to conserve Sharks in Indonesia |
| Descripción del área de estudio | Taman Nasional Perairan Laut Sawu or Savu Sea National Marine Park is located in East Nusa Tenggara Indonesia covering an area of more than three million hectares. The hallmark of the marine park is its unique feature as a migratory area for whale species and is a home for a variety of marine megafauna. It houses 11 whale species, 7 dolphin species, 5 manta, and Turtle species and includes two sunfish and dugongs. |
| Descripción del diseño | The sampling collection event summarized the collection event across 4 locations in Savu Sea waters. We collected surface seawater samples in 5 sites from each location. eDNA sample from sea water sample were collected across 4 designated locations across Sawu Sea, with 5 sites in each location. 2 liter of sea water sample were collected with sterile nalgene bottle from sea surface in every site. Water sample than filtered through a 0.22-133 micron Sterivex™ filter. eDNA samples and blanks will be extracted using the DNeasy Blood & Tissue Kit (QIAGEN, Hilden, 143 Germany). Extracted DNA was then sent to sequencing facility for NGS metabarcoding method using Illumina platform. |
Personas asociadas al proyecto:
Métodos de muestreo
The sampling collection event summarized the collection event across 4 locations in Savu Sea waters. We collected surface seawater samples at 5 sites from each location. eDNA samples from seawater sample were collected across 4 designated locations across the Sawu Sea, with 5 sites in each location. 2 liters of seawater samples were collected with sterile Nalgene bottle from the sea surface in every site. Water sample than filtered through a 0.22-133 micron Sterivex™ filter. eDNA samples and blanks will be extracted using the DNeasy Blood & Tissue Kit (QIAGEN, Hilden, 143 Germany). Extracted DNA was then sent to a sequencing facility for the NGS metabarcoding method using the Illumina platform.
| Área de Estudio | Taman Nasional Perairan Laut Sawu or Savu Sea National Marine Park is located in East Nusa Tenggara Indonesia covering an area of more than three million hectares. The hallmark of the marine park is its unique feature as a migratory area for whale species and is a home for a variety of marine megafauna. It houses 11 whale species, 7 dolphin species, 5 manta, and Turtle species and includes two sunfish and dugongs. For this study, we specifically choose areas from Solor, Rote, Adonara, Manggarai, and West Manggarai to collect the water samples. The areas have never been studied before and hence the biodiversity information from the area will important for biodiversity management and conservation |
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Descripción de la metodología paso a paso:
- 1. For each of the sampling sites, one water samples were collected an homogenized 2. DNA was extracted from each of the samples with a modified protocol based on PowerMax soil kit (MoBio, now Qiagen) 3. For each DNA extract 1 µl, the COI region was amplified in triplicates with the primers mlCOIintF and jgCOIR, using Amplitaq Gold. 4. All PCR products were built into Illumina sequencing libraries with the TruSeq PCR free kit. 5. Sequencing of the 10 samples was done with the Illumina MiSeq platform using the V2 kit 250 bp paired end sequencing. 6. Paired reads were demultiplexed and analyzed with Metaworks https://github.com/terrimporter/MetaWorks using cutadapt, vsearch and dada2, and vsearch. 7. Sequences were annotated by comparison with the Ribosomal Database classifier v2.13 (RDP classifier)
Metadatos adicionales
| Identificadores alternativos | bbb81807-fc4c-4e4e-bc1c-2caee84a4a06 |
|---|---|
| https://cloud.gbif.org/asia/resource?r=edna_id_2022 |