説明
The elasmobranch targeted eDNA project aim to enumerate elasmobranch diversity using eDNA Metabarcoding across eastern Indonesia. A primary goal of the project is to produce a comprehensive database on shark species occurrences in areas around Savu seas, Nusa Tenggara to support current government efforts to save sharks Population. To date we have collected more than 10 water samples from MPA areas around Savu Seas using tools designed to collect water samples (sterivex, and Niskin water sampler). The project is a collaboration between Biodiversitas Indonesia and Misool Foundation. As of now, the DNA reads collected from the samples have no Elasmobranch detected, nonetheless we still publish the taxa that were detected from the samples while working on possible eDNA marker that most suitable to detect elasmobranch species.
データ レコード
この オカレンス（観察データと標本) リソース内のデータは、1 つまたは複数のデータ テーブルとして生物多様性データを共有するための標準化された形式であるダーウィン コア アーカイブ (DwC-A) として公開されています。 コア データ テーブルには、2,063 レコードが含まれています。
拡張データ テーブルは1 件存在しています。拡張レコードは、コアのレコードについての追加情報を提供するものです。 各拡張データ テーブル内のレコード数を以下に示します。
この IPT はデータをアーカイブし、データ リポジトリとして機能します。データとリソースのメタデータは、 ダウンロード セクションからダウンロードできます。 バージョン テーブルから公開可能な他のバージョンを閲覧でき、リソースに加えられた変更を知ることができます。
バージョン
次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。
引用方法
研究者はこの研究内容を以下のように引用する必要があります。:
Anggoro A (2023). Savu Sea-Indonesia eDNA Dataset. Version 1.97. BIONESIA. Occurrence dataset. https://cloud.gbif.org/asia/resource?r=edna_id_2022&v=1.97
権利
研究者は権利に関する下記ステートメントを尊重する必要があります。:
パブリッシャーとライセンス保持者権利者は BIONESIA。 This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.
GBIF登録
このリソースをはGBIF と登録されており GBIF UUID: bbb81807-fc4c-4e4e-bc1c-2caee84a4a06が割り当てられています。 Participant Node Managers Committee によって承認されたデータ パブリッシャーとして GBIF に登録されているBIONESIA が、このリソースをパブリッシュしました。
キーワード
Occurrence; Observation
連絡先
- データ提供者
- Program Development Consultant
- Jl. Buluh Indah No.122, Pemecutan Kaja, Kec. Denpasar Utara
http://orcid.org/https://orcid.org/0000-0002-5482-4123
- データ提供者
- Program Development Consultant
- Jl. Buluh Indah No.122, Pemecutan Kaja, Kec. Denpasar Utara
- データ所有者 ●
- 連絡先
- Director
- Jl. Buluh Indah No.122, Pemecutan Kaja, Kec. Denpasar Utara, Kota Denpasar, Bali 80118
- データ提供者
- Jl. Buluh Indah No.122, Pemecutan Kaja, Kec. Denpasar Utara, Kota Denpasar, Bali 80118
地理的範囲
The Savu Sea is a small sea within Indonesia named for the island of Savu (Sawu) on its southern boundary. It is bounded by Savu and Rai Jua to the south, the islands of Rote and Timor (split between East Timor and Indonesia) to the east, Flores and the Alor archipelago to the north/northwest, and the island of Sumba to the west/northwest. Between these islands, it flows into the Indian Ocean to the south and west, the Flores Sea to the north, and the Banda Sea to the northeast.
| 座標（緯度経度） | 南 西 [-10.509, 120.234], 北 東 [-8.418, 123.816] |
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生物分類学的範囲
DNA sequences from water samples will be identified to species whenever possible. When sequences were unable to be assigned to a taxonomic name, a dedicated OTU (Operational Taxonomic Name)/occurenceID will be given to the sequences
| Kingdom | Bacteria |
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プロジェクトデータ
The proposal aims to enrich existing 2000 coral reef fish database species found in Eastern Indonesia, by particularly focusing on elasmobranch, one of the species that are heavily targeted in global fisheries industries. Major activities will include comprehensively list the group using the most updated eDNA metabarcoding technique and train conservationists to use molecular data for conservation purposes. An important target for the project is to develop a practical field guidebook to collect and analyze eDNA data for research and conservation purposes. The combined activities will contribute massively for education, research and marine conservation activities in the region.
| タイトル | Sawu-Sea Indonesia eDNA Metabarcoding Project |
|---|---|
| 識別子 | BIFA6_021 |
| ファンデイング | Biodiversity Information Fund for Asia (BIFA) Miscellaneous awards given to BIONESIA and Misool Foundation to conserve Sharks in Indonesia |
| Study Area Description | Taman Nasional Perairan Laut Sawu or Savu Sea National Marine Park is located in East Nusa Tenggara Indonesia covering an area of more than three million hectares. The hallmark of the marine park is its unique feature as a migratory area for whale species and is a home for a variety of marine megafauna. It houses 11 whale species, 7 dolphin species, 5 manta, and Turtle species and includes two sunfish and dugongs. |
| 研究の意図、目的、背景など（デザイン） | The sampling collection event summarized the collection event across 4 locations in Savu Sea waters. We collected surface seawater samples in 5 sites from each location. eDNA sample from sea water sample were collected across 4 designated locations across Sawu Sea, with 5 sites in each location. 2 liter of sea water sample were collected with sterile nalgene bottle from sea surface in every site. Water sample than filtered through a 0.22-133 micron Sterivex™ filter. eDNA samples and blanks will be extracted using the DNeasy Blood & Tissue Kit (QIAGEN, Hilden, 143 Germany). Extracted DNA was then sent to sequencing facility for NGS metabarcoding method using Illumina platform. |
プロジェクトに携わる要員:
収集方法
The sampling collection event summarized the collection event across 4 locations in Savu Sea waters. We collected surface seawater samples at 5 sites from each location. eDNA samples from seawater sample were collected across 4 designated locations across the Sawu Sea, with 5 sites in each location. 2 liters of seawater samples were collected with sterile Nalgene bottle from the sea surface in every site. Water sample than filtered through a 0.22-133 micron Sterivex™ filter. eDNA samples and blanks will be extracted using the DNeasy Blood & Tissue Kit (QIAGEN, Hilden, 143 Germany). Extracted DNA was then sent to a sequencing facility for the NGS metabarcoding method using the Illumina platform.
| Study Extent | Taman Nasional Perairan Laut Sawu or Savu Sea National Marine Park is located in East Nusa Tenggara Indonesia covering an area of more than three million hectares. The hallmark of the marine park is its unique feature as a migratory area for whale species and is a home for a variety of marine megafauna. It houses 11 whale species, 7 dolphin species, 5 manta, and Turtle species and includes two sunfish and dugongs. For this study, we specifically choose areas from Solor, Rote, Adonara, Manggarai, and West Manggarai to collect the water samples. The areas have never been studied before and hence the biodiversity information from the area will important for biodiversity management and conservation |
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Method step description:
- 1. For each of the sampling sites, one water samples were collected an homogenized 2. DNA was extracted from each of the samples with a modified protocol based on PowerMax soil kit (MoBio, now Qiagen) 3. For each DNA extract 1 µl, the COI region was amplified in triplicates with the primers mlCOIintF and jgCOIR, using Amplitaq Gold. 4. All PCR products were built into Illumina sequencing libraries with the TruSeq PCR free kit. 5. Sequencing of the 10 samples was done with the Illumina MiSeq platform using the V2 kit 250 bp paired end sequencing. 6. Paired reads were demultiplexed and analyzed with Metaworks https://github.com/terrimporter/MetaWorks using cutadapt, vsearch and dada2, and vsearch. 7. Sequences were annotated by comparison with the Ribosomal Database classifier v2.13 (RDP classifier)
追加のメタデータ
| 代替識別子 | bbb81807-fc4c-4e4e-bc1c-2caee84a4a06 |
|---|---|
| https://cloud.gbif.org/asia/resource?r=edna_id_2022 |