說明
We provide occurrence data of black flies (Diptera: Simuliidae) from Indonesia, Malaysia, Thailand, and Vietnam with corresponding DNA derived data. The black flies were collected by Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya, Malaysia, except black flies from Thailand. DNA barcodes based on mitochondrial cytochrome c oxidase subunit I (COI) gene generated from the black fly samples are used to establish a database in GBIF. This project (BIFA6_017) is funded by the GBIF-BIFA program.
資料紀錄
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 284 筆紀錄。
此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Low V L, Ya'cob Z, Putt Q Y, Izwan-Anas N, Hew Y X (2023). Black Flies (Diptera: Simuliidae) of South East Asia. Version 1.10. Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya. Occurrence dataset. https://cloud.gbif.org/asia/resource?r=black_flies_of_indonesia_malaysia_and_vietnam&v=1.10
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: c80987f7-f87a-4ae3-a2cc-ccd59bc951e8。 Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya 發佈此資源，並經由Participant Node Managers Committee同意向GBIF註冊成為資料發佈者。
關鍵字
Occurrence; Simuliidae; Black Flies; Indonesia; Malaysia; Thailand; Vietnam; COI
聯絡資訊
- 出處 ●
- 連絡人
- Principal Investigator
- Level 2, High Impact Research (HIR) Building, Universiti Malaya
- 連絡人
- Co-Principal Investigator
- Level 2, High Impact Research (HIR) Building, Universiti Malaya
- 元數據提供者 ●
- 出處 ●
- 連絡人
- Graduate Student
- Level 2, High Impact Research (HIR) Building, Universiti Malaya
- 元數據提供者 ●
- 出處 ●
- 連絡人
- Graduate Student
- Level 2, High Impact Research (HIR) Building, Universiti Malaya
- 元數據提供者 ●
- 出處 ●
- 連絡人
- Graduate Student
- Level 2, High Impact Research (HIR) Building, Universiti Malaya
- 連絡人
- Co Principal Investigator
- Level 2, High Impact Research (HIR) Building, Universiti Malaya
地理涵蓋範圍
The black fly samples were collected from different localities in Indonesia, Malaysia, Thailand and Vietnam.
| 界定座標範圍 | 緯度南界 經度西界 [-10.401, 94.746], 緯度北界 經度東界 [23.08, 141.592] |
|---|
時間涵蓋範圍
| 起始日期 / 結束日期 | 2010-12-29 / 2021-06-01 |
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計畫資料
This project will digitize black flies from Southeast Asia and seek to address taxonomic issues via DNA barcoding and metabarcoding approaches, providing a way forward for establishing a DNA barcode library for the family Simuliidae and long-term improvements and additions to black fly information in Southeast Asia.
| 計畫名稱 | DNA barcoding and taxonomic classification of black flies in Southeast Asia |
|---|---|
| 辨識碼 | BIFA6_017 |
| 經費來源 | Funded by Ministry of the Environment, Government of Japan; Co-funded by TIDREC, Universiti Malaya, Malaysia |
| 研究區域描述 | Black flies collected from the Oriental Region (particularly Southeast Asia) belong to the genus Simulium. |
參與計畫的人員:
- 研究主持人
- 研究主持人
- 發布者
- 發布者
- 發布者
取樣方法
Black fly samples collection was done at the accessible streams and rivers, whereby these are the breeding habitats for larvae and pupae of black flies. Pupae that attached on substrates such as leaves, stems, twigs, grasses and roots were collected using forceps. Then it will be kept alive individually in vials until adult emergence before being preserved in 80% ethanol for the identification process. The methods of collection and identification followed those of Takaoka (2003) and Adler et al. (2004).
| 研究範圍 | All black fly samples were collected from flowing streams. |
|---|
方法步驟描述:
- The study procedures are as follows: sample collection, sample identification, sample DNA extraction, PCR.
收藏資料
| 蒐藏名稱 | Institute of Biological Sciences (ISB), Faculty of Science, Universiti Malaya, Malaysia |
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| 蒐藏名稱 | Black Fly Gallery, TIDREC, Universiti Malaya, Malaysia |
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| 標本保存方法 | Alcohol |
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額外的詮釋資料
Black fly samples, except from Thailand, of this dataset are deposited in the laboratories of Institute of Biological Sciences and TIDREC, Universiti Malaya, Malaysia. Upon collection from study sites in Indonesia, Malaysia, and Vietnam, the black fly samples were indexed in the format of "Nation_Locality_Stream_# where collection occured_Vial_# where the sample from respective stream kept". For instance, the black fly sample from the first vial of stream 1, Lombok, Indonesia would be indexed as "IN_LB_S1V1". The black fly samples collected are individually preserved in 80% ETOH in a 2.0 mL tube and stored at -20 degree Celsius.
| 替代的識別碼 | c80987f7-f87a-4ae3-a2cc-ccd59bc951e8 |
|---|---|
| https://cloud.gbif.org/asia/resource?r=black_flies_of_indonesia_malaysia_and_vietnam |