Description
We provide occurrence data of black flies (Diptera: Simuliidae) from Indonesia, Malaysia, Thailand, and Vietnam with corresponding DNA derived data. The black flies were collected by Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya, Malaysia, except black flies from Thailand. DNA barcodes based on mitochondrial cytochrome c oxidase subunit I (COI) gene generated from the black fly samples are used to establish a database in GBIF. This project (BIFA6_017) is funded by the GBIF-BIFA program.
Data Records
The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 284 records.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versions
The table below shows only published versions of the resource that are publicly accessible.
How to cite
Researchers should cite this work as follows:
Low V L, Ya'cob Z, Putt Q Y, Izwan-Anas N, Hew Y X (2023). Black Flies (Diptera: Simuliidae) of South East Asia. Version 1.10. Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya. Occurrence dataset. https://cloud.gbif.org/asia/resource?r=black_flies_of_indonesia_malaysia_and_vietnam&v=1.10
Rights
Researchers should respect the following rights statement:
The publisher and rights holder of this work is Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
This resource has been registered with GBIF, and assigned the following GBIF UUID: c80987f7-f87a-4ae3-a2cc-ccd59bc951e8. Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Participant Node Managers Committee.
Keywords
Occurrence; Simuliidae; Black Flies; Indonesia; Malaysia; Thailand; Vietnam; COI
Contacts
- Originator ●
- Point Of Contact
- Principal Investigator
- Level 2, High Impact Research (HIR) Building, Universiti Malaya
- Point Of Contact
- Co-Principal Investigator
- Level 2, High Impact Research (HIR) Building, Universiti Malaya
- Metadata Provider ●
- Originator ●
- Point Of Contact
- Graduate Student
- Level 2, High Impact Research (HIR) Building, Universiti Malaya
- Metadata Provider ●
- Originator ●
- Point Of Contact
- Graduate Student
- Level 2, High Impact Research (HIR) Building, Universiti Malaya
- Metadata Provider ●
- Originator ●
- Point Of Contact
- Graduate Student
- Level 2, High Impact Research (HIR) Building, Universiti Malaya
- Point Of Contact
- Co Principal Investigator
- Level 2, High Impact Research (HIR) Building, Universiti Malaya
Geographic Coverage
The black fly samples were collected from different localities in Indonesia, Malaysia, Thailand and Vietnam.
| Bounding Coordinates | South West [-10.401, 94.746], North East [23.08, 141.592] |
|---|
Temporal Coverage
| Start Date / End Date | 2010-12-29 / 2021-06-01 |
|---|
Project Data
This project will digitize black flies from Southeast Asia and seek to address taxonomic issues via DNA barcoding and metabarcoding approaches, providing a way forward for establishing a DNA barcode library for the family Simuliidae and long-term improvements and additions to black fly information in Southeast Asia.
| Title | DNA barcoding and taxonomic classification of black flies in Southeast Asia |
|---|---|
| Identifier | BIFA6_017 |
| Funding | Funded by Ministry of the Environment, Government of Japan; Co-funded by TIDREC, Universiti Malaya, Malaysia |
| Study Area Description | Black flies collected from the Oriental Region (particularly Southeast Asia) belong to the genus Simulium. |
The personnel involved in the project:
- Principal Investigator
- Principal Investigator
- Publisher
- Publisher
- Publisher
Sampling Methods
Black fly samples collection was done at the accessible streams and rivers, whereby these are the breeding habitats for larvae and pupae of black flies. Pupae that attached on substrates such as leaves, stems, twigs, grasses and roots were collected using forceps. Then it will be kept alive individually in vials until adult emergence before being preserved in 80% ethanol for the identification process. The methods of collection and identification followed those of Takaoka (2003) and Adler et al. (2004).
| Study Extent | All black fly samples were collected from flowing streams. |
|---|
Method step description:
- The study procedures are as follows: sample collection, sample identification, sample DNA extraction, PCR.
Collection Data
| Collection Name | Institute of Biological Sciences (ISB), Faculty of Science, Universiti Malaya, Malaysia |
|---|
| Collection Name | Black Fly Gallery, TIDREC, Universiti Malaya, Malaysia |
|---|
| Specimen preservation methods | Alcohol |
|---|
Additional Metadata
Black fly samples, except from Thailand, of this dataset are deposited in the laboratories of Institute of Biological Sciences and TIDREC, Universiti Malaya, Malaysia. Upon collection from study sites in Indonesia, Malaysia, and Vietnam, the black fly samples were indexed in the format of "Nation_Locality_Stream_# where collection occured_Vial_# where the sample from respective stream kept". For instance, the black fly sample from the first vial of stream 1, Lombok, Indonesia would be indexed as "IN_LB_S1V1". The black fly samples collected are individually preserved in 80% ETOH in a 2.0 mL tube and stored at -20 degree Celsius.
| Alternative Identifiers | c80987f7-f87a-4ae3-a2cc-ccd59bc951e8 |
|---|---|
| https://cloud.gbif.org/asia/resource?r=black_flies_of_indonesia_malaysia_and_vietnam |